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The dissociated cells were centrifuged 10 min at 2000 rpm and resuspended in a volume of 300 µl of culture medium per 50 mg of initial tissue and seeded on a poly-L-lysine / laminin coated 1.5 cultureture wells.
Prewashed high-capacity NeutrAvidin agarose resin (Pierce, Rockford, IL) was used to pull down biotinylated proteins at room temperature for 2 3 h (Pierce; 50 µL dried beads per 1 mg of initial starting material).
The protein pellet was resuspended in distilled water containing 2% sodium dodecylsulfate (SDS) (300 μL/100 mg of initial tissue).
Pellets were extracted again with acetonitrile (10 µl of acetonitrile for each mg of initial tissue) by vortexing for 10 min.
The concentration of the ground extract was adjusted by adding 80% methanol to be equivalent to 32 mg of initial wet weight per milliliter of methanol extract.
The soluble collagen contents of the bDBM and bECM material were determined to be 0.93 ± 0.06 and 0.92 ± 0.06 mgs of collagen per mg of initial dry weight respectively.
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The fungus showed the ability to remove Cr VI) (1.24 mg l−1) and total Cr (7.91 mg l−1) from tannery effluent below the detection level within 18 h and 36 h of incubation, respectively, and ability to accumulate 189.13 mg Cr g−1 of dry biomass within 600 h of incubation from spent chrome effluent [containing 3731.4 mg l−1 of initial Cr III) concentration].
However, at 100 mA depollution becomes more effective from 71 mg l−1 of initial pollutant.
The system resulted in more than 99% gold being recovered within 2 h of operation for 100 mg L−1 of initial gold III) chloride concentration.
ZVI supported on activated carbon heat-treated at 900 °C yields the best phenol removal, reaching over 85% after 3 h, under the conditions tested: 150 mg L−1 of initial phenol concentration, initial pH set at 3, 30 °C, and the stoichiometric amount of hydrogen peroxide for achieving complete mineralization.
With the results of preliminary experiments done by 'one variable at a time method', further experiments have been carried out by a three level full factorial design of Response Surface Methodology (RS M and consequently the optimum conditions for the biosorption of lead have been identified as 6.96 g L−1 of adsorbent dosage, 19.77 mg L−1 of initial adsorbate concentration and 4.4 pH.
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