Sentence examples for mg of human from inspiring English sources

The separation power and sensitivity provided by the nanoLC LTQ enabled identification of ∼4600 phosphopeptide candidates from ∼60 μg COS-7 cell tryptic digest followed by IMAC enrichment and ∼520 tyrosine phosphopeptides from ∼2 mg of human T cells digests followed by phosphotyrosine peptide immunoprecipitation.

RNA extraction yielded an average of 50 60 µg of total RNA from 20 mg of human prostate tissue.

An average of 7 mg of human skin tissues were extracted in Laemmli buffer using the bullet blender according to manufacturer instructions (Next Advance, Averill Park, NY).

Briefly, 10 mg of human orosomucoid was dissolved in 1 mL of 50 mM acetate buffer, pH 5, and incubated with 0.6 IU of immobilized neuraminidase for 3 hours at 37°C.

The medium was allowed to evaporate for approximately 15 min and discs containing either 50 mg of ferric nitrate or 100 mg of human or bovine haemoglobin (Sigma) were placed on the plate.

Briefly, 100 mg of human liver or 5×106 cells were lysed in 1 ml of TRIzol reagent at 22°C for 5 min. Chloroform (200 ml) was added to the lysates, and samples were mixed by vortexing.

For cells stably expressing human GPR119, RINm5f cells were transfected with 5 mg of human-GPR119 cDNA (pCDNA3.1) using Nucleofector Solution V and an Amaxa Nucleofector II (Amaxa Biosystems, Germany).

MCF-7 cells were grown in Eagle's Minimum Essential Medium (EMEM) supplemented with 0.01 mg mL−1 of human recombinant insulin and 10% FCS.

Caco-2 cells (ATCC) were grown on 25 mm glass coverslips and maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 1% non-essential amino acids, 0.01 mg ml−1 of human transferrin, 10 000 U of penicillin per ml, and 10 ng of streptomycin per ml in an atmosphere of 95%air-5air-5%.

From 1 l of autoinduced culture of Rosetta DE3) cells, 100 mg of pure human P2 were routinely obtained (Figure S1).

Heparin-binding proteins were isolated from 500 mg of frozen human HCC homogenized in non denaturing buffer with protease inhibitors (Sigma), clarified by ultracentrifugation and applied to a 5-ml HiTrap heparin column.