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To 600 mg of fresh onion bulb tissue, 600 μL distilled water was added, and either heated for 1 hour at 60 °C, or held for 1 hour at room temperature.
Four hundred mg of fresh onion bulb tissue was crushed with or without 12.5 units purified alliinase using a micro pestle for 20 seconds, and immediately after centrifugation at 15,000 rpm at 4 °C for 1 min, 1 μL of the supernatant was applied to the HPLC (LC-10AT, Shimadzu) equipped with a Senshu Pack ODS column (250 mm × 4.6 mm, 5 μm, Senshu Scientific) and a UV detector (254 nm).
Each reactor was filled with 200 mg of fresh catalyst.
Total RNA was extracted from 100 mg of fresh tissues using TRIzol reagent (Invitrogen).
The experiment was performed using a quantity of 15 mg of fresh prepared sample.
The concentrations are expressed as micrograms of Trolox equivalents per 100 mg of fresh weight.
DNA was extracted from 50 mg of fresh rice leaves using the DNeasy 96 Plant Kit (Qiagen).
Temperature-programmed reduction (TPR) was performed on chemisorption machine (ChemBet 3000, Quantachrom, USA), in which 30 mg of fresh catalyst was loaded in the thermostatic zone.
Primarily, 125 mg of fresh adsorbent was added to 100 ml of 5 mg/L CR solution at pH 7.0 and shaken for 12 h.
The extraction of lipid peroxides was carried out using 500 mg of fresh shoot tissue, 0.3 mL of 10% trichloroacetic acid, and 1 mL of 0.5% TBA.
Approximately 15 mg of fresh leaf of affected R. conglomeratus was placed in 7 mL of N N dimethylformamide (DMF) for 24 h in darkness at 4 °C for extraction (Moran and Porath 1980).
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