Sentence examples for mg of enzyme from inspiring English sources

In all cases, 0.83 mg of enzyme was precipitated.

In another study (Dong et al. 2010), it was reported that the addition of 10 mg BSA per 100 mg of enzyme has resulted in an increase of recovered activity from 24 to 82 %.

Specific activity is defined as the number of units per mg of enzyme.

The specific activity was determined as μmol of product formed/min per mg of enzyme.

It required 6,661 immature seeds for Kwak et al. [ 9] to obtain 0.086 mg of enzyme described as partially pure.

One unit of enzyme was defined as 1 μmol of p-nitrophenyl released per mg of enzyme per minute under our experimental conditions.

The reactions were performed using microplates as follows: 50 mM Tris HCl pH 8.0, 20 mM L-Gln, 0.13 mM NADH, 0.5 U GDH (diluted in 50 mM sodium phosphate pH 7.4; 50% glycerol) and 0 mg to 0.09 mg of ScASNase1 enzyme or the commercial enzyme EcASNase2 (Prospec–Tany, Israel), which was used as a positive control.

Briefly, the mixture containing 50 ml (10 mg) crude of enzyme solution, 1.83 ml Tris-HCl buffer (0.1 M, pH 8.0) and 100 μl reduced glutathione (GSH, 50 mM, Sigma).

APPD production from ginseng root extract increased when the enzyme concentration was increased; however, the amount of additional APPD production per mg of added enzyme significantly decreased above 8 mg ml−1 DT-bgl (Fig. 1b), indicating that the optimal enzyme concentration was 8 mg ml−1.

The recombinant enzyme was purified by a one-step Ni-sepharose affinity chromatography protocol, which typically provides ∼200 mg of homogeneous enzyme per liter of culture.

Single-step purification using immobilized metal affinity chromatography yielded 8 mg of pure enzyme per liter of culture, which is fully active on a range of protein and peptide substrates.