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We isolated the total genomic DNA from approximately 100 mg of dried powder from each sample using the cetyl trimethylammonium bromide method.
Total P was extracted from approx. 10 to 20 mg of dried tissue by digesting in 3 ml HNO3 at 100°C for 10 min.
Dried samples were weighed out and extracted as previously described, with the solvent volumes adjusted to maintain a ratio of 1 mL of solvent per 1 mg of dried cellular material.
Similarly 10 mg of dried different extracts was dissolved in 10 mL of specific solvent for the preparation of stock solution of sample to give concentration of 1 mg/mL.
Genomic DNA was extracted from 0.1 10 mg of dried herbarium specimens using the DNeasy Plant Mini kit (Qiagen) or the Nucleospin Plant II mini kit (Macherey-Nagel) following the manufacturers' instructions.
After the algal pellets were freeze dried, 50 mg of dried algal biomass was homogenised and then extracted with n-hexane thrice.
Around 50 mg of dried plant tissue was used for starch quantification, employing a total starch assay procedure kit (Megazyme, Bray, Ireland).
DA/SBA-15 Composites: 100 mg of dried SBA-15 were suspended in 40 mL of anhydrous toluene in a round bottomed flask under nitrogen.
About 50 mg of dried sediment weighed in a tin capsules was analyzed using automated continuous-flow isotope ratio mass spectrometry (Preston and Owens 1983).
1 mg of freeze-dried four polysaccharides were mixed with 150 mg of dried potassium bromide (KBr), and pressed into disk for the analysis.
50 mg of dried plant tissues were ground up and digested in 1 mL of concentrated nitric acid for 2 3 d at room temperature.
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