Sentence examples for mg of cellular from inspiring English sources

HGF was immuno-precipitated from 2 mg of cellular protein and probed for HGF by Western blot analysis.

The reaction was started by adding 125 µl of sample, and the rate of formation of CDNB-GSH conjugate was monitored at 340 nm for 6 min. GST activity was calculated using the extinction coefficient of 9.6 mM−1 cm−1, and expressed as µmol of CDNB-GSH conjugate formed per min per mg of cellular protein.

Twelve and a half million promastigotes (about 25 µg of total protein) suspended in 18.75 µl DIG buffer (25 mM Tris, pH 7.5, 1 mM EDTA, 0.6 M sucrose) containing 50 µg ml−1 proteinase K (PK), were permeabilized by addition of 6.25 µl of prediluted digitonin (Calbiochem) to final concentrations of 0 2 mg of digitonin per mg of cellular protein.

CICP release was then reported as ng per mg of cellular protein.

The uptake level was expressed as ng FITC-polypeptide associated with 1 mg of cellular protein.

The S12 extracts had 33 mg of cellular protein per mL.

GLC4 cells have a slightly lower although not significant GSH content of 9.9±2.2 μg mg of total cellular protein compared with GLC4-Adr cells with a cellular GSH content of 11.1±6.1 μg mg−1 (P=0.75).

Assuming that 1 mg of total cellular protein represents about 3×10^9 cells, it may be calculated that each cell contains about 8,500 acetylase subunits.

For immunoprecipitation (IP), 0.5∼1.0 mg of total cellular protein was incubated with primary antibody at 4°C overnight, followed by the addition of Protein A/G-Sepharoses and additional incubation at 4°C for 1 h, then resolved by Tris-Tricine gel or SDS-PAGE.

Approximately 125 mg of soluble cellular extract were loaded on the column.

100 mg of total cellular protein was loaded on polyacrylamide gels and separated by standard 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis.