Sentence examples for mg of cell from inspiring English sources

For immunoprecipitation of over-expressed proteins in fibroblasts, 4 mg of cell lysate was incubated o/n with protein G beads coated with anti-FLAG antibody or GFP Trap_MA beads from Chromotek.

In YPD, YPSuc and YPInu media, total inulinase activities were 600, 650 and 1920 U mg of cell dry weight-1 at 30°C, respectively, and 1140, 1290 and 1390 U mg of cell dry weight-1 at 45°C, respectively.

10 to 20 mg of cell walls was suspended in 1 ml of 1.6 N NaOH.

Briefly, 45 mg of cell sediment were suspended in Tris-HCl (pH 7.4) buffer containing 1% sodium dodecyl sulfate.

1 mg of cell lysates were incubated with 100 µl of anti-albumin Ab (Abcam -conjugated SephAbcam -conjugated µl PBSepharosefor overnight.

A total of 6 mg of cell lysate per cell state (different time points following FGF-2 stimulation) was digested with trypsin and this peptide digest was subsequently enriched for tyrosine phosphorylated peptides.

The specific activity of GDH is expressed as μmol of substrate reduced/min/mg of cell protein, and represents the averages for at least three cell preparations.

The results of IL-6 and CCL5 measurements were then expressed as pg of IL-6 or CCL5/mg of cell protein lysates.

Immunoprecipitation was performed in a 1.5-ml Eppendorf tube and 1 μg of antibody/mg of cell lysate was used.

A small portion (5 10 mg) of cell/tumor lysate from all treatment groups was homogenized in Laemmli buffer that consisted of 0.5 M Tris base, 10% sodium dodecyl sulfate (SDS), 2- β-mercaptoethanol, and glycerol containing 100  μM sodium orthovanadate, a protease inhibitor [ 35].

The TAK1 complexes were pulled down from 1 mg of cell lysate obtained from TAB1 reconstituted MEFs.