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The most favourable conditions under the experimental range studied were found as 70 °C, 20% ethanol, and 10 g of CO2 min−1, for which EY = 0.48%, TPC = 57.22 mg of gallic acid equivalents (GAE) g−1 of extract, PC-HPLC = 119.46 mg g−1 of extract, and AA = 49.74 mg of ascorbic acid equivalents (AAE) g−1 of extract.
The plasma bioavailability of vitamins C and E were determined in normal fasting subjects, with eight subjects for vitamin C and ten subjects for vitamin E. In a random crossover design, the subjects consumed either 500 mg of ascorbic acid or 420 mg of vitamin E acetate alone (control), or combined with 2 oz of two different Aloe preparations (a whole leaf extract, or an inner fillet gel).
The results are expressed as mg of ascorbic acid/kg of honey.
The mean value is expressed as mg of ascorbic acid equivalent antioxidant content per 100 g of honey.
Subsequently, 50 mg of ascorbic acid was rapidly added and the mixture maintained at 55 °C for 30 min, before being allowed to cool to room temperature.
Samples (1 L each) were collected in silanized amber glass bottles containing 50 mg of ascorbic acid to quench residual chlorine and 1 g of sodium azide to prevent microbial activity.
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The antioxidant activity was expressed as the number of mg equivalents of ascorbic acid per gram of dry plant weight i.e., mg AAE/g DW [ 7, 10].
**: Ascorbic acid equivalent antioxidant activity: milligram of ascorbic acid equivalents per 100 g of plant dry weight (mg AA/100 g DW).
AA and DHAA contents were expressed as mg of L-ascorbic or L-dehydroascorbic acid per 100 g of fresh weight.
To 0.5 ml of each cell lysate, 0.5 ml of freshly prepared 0.15 M sodium acetate buffer (pH 4.6) containing 16 pg of each SI-EM and 2 mg of L-ascorbic acid was added.
In guinea pigs, a 'megadose' of 150 mg per day of ascorbic acid decreased the severity of surgically induced knee OA [ 59, 60] but increased severity of spontaneous OA [ 61], despite the ability of ascorbic acid to increase cartilage collagen content.
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