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Plants received total amount of 7.5 mg from each compounds as low concentration (TW + LC) and 37.5 mg as high concentration (TW + HC) for 33 days of growth.
RNA (1 mg) from each sample was reverse-transcribed using 25 U of superscript II reverse transcriptase (Invitrogen).
A small portion (<10 mg) from each tissue of a fish were pooled with 200 µL HBSS and stored at −80°C for RNA isolation.
A constant amount of bone powder (200 mg) from each sample (Figure 1) was digested with proteinase K. DNA was extracted using Centricon 30.000 MWCO ultra-filtration columns (Millipore, Billerica, MA, USA) followed by a silica based purification method (Qiagen, Valencia, CA, USA) to effectively remove PCR inhibitors [27].
Meniscal explants (approximately 100 mg from each group) were frozen immediately in liquid nitrogen.
Lung tissue (200 mg) from each group of mice was homogenized with lysis buffer (1000 μl RIPA).
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Rock powder of ~150 mg weight from each sample was prepared and packed in a gelatin capsule for measurements.
In brief, 40 tumor specimens (100 150 mg each) from each group were washed in PBS and minced into small pieces using bistouries.
Briefly, 40 tumor specimens (100 150 mg each) from each group were washed in PBS and minced into small pieces using bistouries.
Then, an aliquot of 200 mg fat from each sample was dissolved in 5 ml petroleum ether.
Equal amounts of protein (2.5 mg) extracted from each cell population were mixed and digested with trypsin.
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