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In both, the P-CF (cell-free membrane protein expression as precipitate) mode generating initial aquaporin precipitates as well as in the D-CF (cell-free membrane protein expression in presence of detergent) mode, generating directly detergent solubilized samples, we were able to obtain mg amounts of protein per ml of cell-free reaction.
In addition to biopharmaceuticals, structural genomic initiatives also require milligram (mg) amounts of protein for three-dimensional (3D) structure representations.
The large mg amounts of dye may be responsible for the lack of dynamical lymph motion observed using these devices.
To collect mg amounts of proteins for FPLC separation, two successive extractions were performed with 0.2 M CaCl2 and 2 M LiCl [ 14].
(2) Bypassing these drawbacks associated with heterologous expression, Tanaka et al. developed a protease-deficient C. perfringens strain 13 expression system and were able to purify mg amounts of homogeneous ColH (Tamai et al. 2008; Tanaka et al. 2008).
To demonstrate that hRad9 protein in breast cancer cells is in phosphorylated forms, 100 mg amounts of frozen tissue were extracted as previous in the presence of protease inhibitors (protease inhibitor cocktail, set III; EMD Biosciences Inc, CA, USA).
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The optimization conditions were determined as pH 9, 1 mg amount of iodogen and 30 minutes of reaction time for SubPc; pH 7, 1 mg amount of iodogen and 45 minutes of reaction time for Zn II Pc.
A 30 mg amount of each sample was mixed with 1.0 mL of a 0.85 N HCl solution containing 24000 U of porcine pepsin per mL.
A 156 mg amount of YCl3, 70 mg of YbCl3, and 8 mg of ErCl3 were mixed with 15 mL of 1-octadecene and 12 mL of oleic acid and heated to 120 °C for half an hour.
A 791 mg amount of the monomer NIPAM and 161 mg of the cross-linker BIS were dissolved in 200 mL of water in a 250 mL three-neck round-bottom flask.
For exchange transfusions, the mg amount of iron removed was calculated based on the volume of packed red blood cells removed (1 ml red blood cells removed equals 1·08 mg iron).
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