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To investigate the 4p15.2 allelic status of NBs with SLIT2 methylation, we typed 13 methylated tumours for loss of heterozygosity (LOH) at D4S1546 that maps close to SLIT2.
To investigate the 4p15.2 allelic status of Wilms' tumours with SLIT2 methylation, we typed six methylated tumours for LOH at D4S1546.
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To test the possible effects of dmtA and tmdA mutations, or over-expression of dmtA, on DNA methylation, we performed two types of experiments.
After grouping the results according to each methylation type, we concluded that there were substantial levels of the three types of methylation (CG, CNG, and CNN) in that region under non-water stress conditions (Fig. 1A).
To examine the effect of DNA methylation clustering, we generated two types of genomes.
To account for the variations in global methylation levels we adopted two types of normalization: the first normalized by the total amount of DNA methylation in the sample and the second was based on the expression of DNMT3b measured via RT-qPCR.
We observed some quite strong associations between the mRNA and methylation sub-types and mutations in selected genes.
DNA methylation, one type of epigenetic change, is the reversible addition of a methyl group to cytosine nucleotides.
To make the result more closer with the sample's actual methylation level, we calculated the methylation rate by the following formula: total rate of methylation = Total number of methylation bands (Type I + Type II + Type III)/Total number of amplified bands (Type II + Type III + Type IV) × 100%.
Second, the full methylation sites (type III) occur more frequently than hemi-methylation sites (type II) in the heart, liver and lung.
First, in our study the hemi-methylation sites (type II) occur more frequently than full methylation sites (type III) in the muscle, spleen, kidney and stomach.
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