Nevertheless, the results only indicated a low methylation trend in migraine versus control group (total average methylation level: 8.41 % ±1.92%% vs. 9.90 % ± 3.88 %, p = 0.197).
However, our results showed that no significant difference of methylation of each examined CpG unit or site within the region (−300 to 205) was observed between patients with migraine and healthy controls except for a low average methylation trend in migraine group.
If at least two CpG sites showed the same methylation trend in a given loci, they configure an age-modified region.
David Jones presented data on the methylomic landscape of medulloblastoma and discussed different techniques to determine methylation trends in the genome.
Among 13 detected CpG sites or units at RAMP1 promoter region, there were no significant differences between the migraine and control groups, but indicating a low methylation trend overall in migraine group (total average methylation level: 8.41 % ±1.92%% vs. 9.90 % ± 3.88 %, p = 0.197).
A low methylation trend overall was presented in migraine subjects, and two CpG units were observed to link with positive migraine family history and female migraine, respectively.
Even though we found no significant differences of DNA methylation pattern between migraine patients and healthy controls, a low methylation trend overall was presented in migraine group, and two CpG units were observed to link with positive migraine family history and female migraine, respectively.
At this point, it is pertinent to clarify that the methylation trends shown in Figures 3 and 5 reflect an average behaviour of all cytosine positions grouped in each gene region and thus may not necessarily match the epigenetic situation of individual cytosine residues like those depicted in Figure 6.
High KLLN promoter methylation was associated with an increased age-adjusted cumulative risk of thyroid cancer in CS/CSL males, whereas the association appears reversed in women, where KLLN promoter methylation trended with decreased cumulative risk of thyroid cancer.
Unlike the consensus methylation trends of the ALVEs regions 1 and 2 (Figure 3A and 3B), the promoter region of TVB shows tissue-specific methylation patterns (Figure 7) similar to the promoter region 3/4 in ALVEs (Figure 3C).
However, when the analysis was restricted to girls with urinary concentrations of BPA below the limit of detection (N = 12) compared to girls characterized as BPA-high (N = 13), a decreasing, yet statistically non-significant, trend in DNA methylation in all candidate CpG sites was observed in all 4 genes.
