Sentence examples for methylation in sorted from inspiring English sources

It is interesting to note that the level of methylation in sorted CD25hi and lo, CD25lo derived, populations converges and levels off at a partial demethylation around 60%.

Interestingly, 38% of 794 age-modified CpG sites identified in this study showed homogeneous DNA methylation in sorted leukocytes, granulocytes and peripheral blood mononuclear cells from healthy adults; suggesting that at least these age-modified CpG sites may not be lineage specific and that it is unlikely that the detected age effects would be a result of differences in cell composition.

Moreover, to avoid the confounding effects of CpG sites that are differentially methylated among leukocyte populations due to cell lineage (cell specific), the 853 age-modified CpG sites were contrasted against a list of 2,228 CpG sites with significant differential DNA methylation in sorted leukocytes [ 34], which serve as cell-type classifiers.

Furthermore, to avoid the confounding effects of CpG sites that are differentially methylated among leukocyte populations (cell-type specific), all age-modified CpG sites were contrasted against a list of 2,228 CpG sites with significant differential DNA methylation in sorted leukocytes [ 34] that serve as cell-type classifiers.

Therefore, further studies addressing methylation differences in sorted cells during gestation, at full-term birth, and later in life may provide empirical data necessary to account for these confounders, as suggested previously by Houseman and colleagues [ 87].

WIF1 and HDAC1 showed similar levels of gene methylations in all sorted subpopulations and MMO cells, but lower levels for parental SUM159 cells.

The DNA methylation landscape of ORMDL3 in sorted blood leukocytes showed that ORMDL3 was significantly less methylated in T lymphocytes, especially CD8+ T-cells (Fig.  6 and Supplementary Material, Table S6).

Predicted age, referred to as DNA methylation age, correlates with chronological age in sorted cell types (CD4 T cells, monocytes, B cells, glial cells, neurons), tissues and organs including whole blood, brain, breast, kidney, liver, lung, saliva [ 19], and even prenatal brain samples [ 21].

To further explore the landscape of DNA methylation in the 17q12-21 asthma-susceptibility locus, we measured DNA methylation in 64 CpG sites spanning from IKZF3 to GSDMA in sorted leukocytes from six healthy male blood donors (n = 60) using the Infinium 450K assay (Supplementary Material, Table S6) (26).

Figure 6 shows patterns of a class in sorted order.

The data showed 12.6-fold higher expression of C46 mRNA in sorted EGFP-positive cells from M05189 relative to sorted EGFP-negative cells.