Sentence examples for methylation in somatic from inspiring English sources

Here we show that G9a is not required for anchoring of DNMT3A/3B to nucleosomes in methylated chromatin regions and for maintenance of DNA methylation in somatic cells.

We have shown that DNMT3A/3B remain bound to methylated chromatin regions even in the absence of G9a indicating that these enzymes do not require G9a for their presence at silent methylated domains and they can faithfully maintain DNA methylation in somatic cells independent of G9a.

However, CpG O/ E has been linked to empirically determined levels of DNA methylation in somatic tissues in insects, suggesting that many genes are universally methylated in germlines and soma (Foret et al. 2009; Xiang et al. 2010).

Weak CpG island promoters are distinct, as they are preferential targets for de novo methylation in somatic cells.

As results, in mature sperm H19 ICR shows biallelic methylation instead of paternal specific methylation in somatic cells.

On H19 imprinting control region (ICR), one of the mechanisms regulating the paternal allelic specific silence is DNA methylation in somatic cells throughout the individual's whole life.

Therefore, our findings clearly indicate that, different from de novo methylation during the early stages of embryogenesis, DNA methylation recovery in somatic cells following 5-Aza-dC treatment was mediated by DNMT1, and not by the conventional de novo DNA methyltransferase, DNMT3B.

However, as methylation distribution in somatic cell is bimodal (i.e., heavily biased towards methylated or unmethylated patterns), Pearson's correlation analysis can confound interpretation of reproducibility; thus the Bland-Altman method is more suitable for this purpose.

While an essential role of G9a in directing de novo DNA methylation in ES cells has been established, whether it plays a similar role in maintenance of DNA methylation patterns in somatic cells remains unclear.

These findings are in generally consistent with the previous reports that have compared methylation profiles in somatic cells, iPS cells, and ES cells [23], [31], [32].

The endogenous ALF gene was characterized by RACE and RT-PCR for transcription start site usage, and by sodium bisulfite sequencing to determine its methylation status in somatic and oocyte tissues.