Compared to phosphorylation, which strongly reduces methylation if in proximity of SET7/9 target lysine residues [ 12], PARylation is a much more bulky modification with more negative charges and therefore also inhibited histone methylation by SET7/9.
Given previous evidence that KS is associated with skewed XCI in peripheral blood, we next assessed allelic patterns of DNA methylation in the proximity of a polymorphic repeat (CAG n in the androgen receptor (AR) gene.
The allelic X-Chromosome Inactivation (XCI) ratios of both tissues of the 47,XXY sample were determined by assessing DNA methylation in the proximity of a polymorphic repeat (CAG n in the human androgen receptor (AR) gene, as described previously., In brief, 50 ng of genomic DNA was incubated with HpaII, MspI or water in triplicate.
Since multiple RNA methylation residuals may locate in proximity and cannot be effectively differentiated with peak calling procedure, they can appear as a single broad methylation site in the peak calling result from MACS [ 27] or exomePeak [ 19].
Among them, a comprehensive study showed cytosine-guanine dinucleotide (CpG) DNA methylation variation in BD patients in proximity of the BDNF SNP rs6265; exonic CpG methylation was associated with the valine variant of rs6265 in a sample of 105 postmortem brains (Mill et al. 2008).
The frequency of methylation associated SNPs in Alu Y/S elements exhibited a gradient pattern suggestive of linear spreading, being higher in proximity to methylation associated SNP clusters and lower closer to CpG islands.
Biological pathway enrichment among genes in proximity to methylation changes associated with GDM.
We omitted array probes from statistical analyses that contained common genetic variants that likely influence DNA binding (Methods), but DNA methylation levels may be mediated by genetic variants in proximity, that is, represent DNA methylation quantitative trait loci (met-QTLs).
In vitro differentiation of ES cells is known to coincide with de novo methylation at over 300 CpG-poor regions that are in proximity to gene promoters [ 25].
