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Previous studies have been focused on imprinted genes with DNA methylation in poor quality human sperms.
However, these studies interrogated for the most part imprinted loci with DNA methylation in poor quality human sperms.
Previous studies of DNA methylation in poor quality human sperm interrogated only imprinted loci, measuring methylation of sequences in only one or two genes [25] [27].
Previous studies have been focused on imprinted genes with DNA methylation in poor quality human sperms [6] [8] and conceptuses conceived using ART (from infertile parents) [22].
We are not aware of data describing DNA methylation in the human germ line at this date; however, the DMR in MEST at which we found elevated DNA methylation in poor quality sperm is reportedly unmethylated in the male germ line by week 24 of gestation [45].
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However, more recent studies report loss of L1 methylation in regard to poor prognosis and survival in melanoma patients [ 55, 56].
In advanced NSCLC, Kaplan Meier analysis has shown a strong correlation between BRMS1 promoter methylation in plasma and poor OS, while multivariate analysis has shown that BRMS1 promoter methylation has a statistical significant influence on both patients' PFS and OS.
In operable NSCLC, Kaplan Meier analysis has shown a strong correlation of BRMS1 promoter methylation in plasma and poor DFI and OS, and these results were further verified by multivariate analysis.
Several earlier studies with one exception (Yang et al, 2004) failed to find a statistical correlation between RASSF1A methylation in tumours and poor outcome (Astuti et al, 2001; Harada et al, 2002; Banelli et al, 2005; Michalowski et al, 2008).
At this sequence we also observed greater DNA methylation in samples with poorer semen parameters (Figure 1).
Concurrent promoter methylation in NEUROG1 and CDKN2A (p16) was associated with poor DFS in stages II and III colorectal cancer patients, and CHFR promoter methylation indicated poor prognosis in stage II colorectal cancer patients [ 4, 6].
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