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We investigated the association between both epigenetic marks and compared the results with LINE-1 and Alu methylation, often used as surrogates for global DNA methylation in monitoring studies, determined with PCR-pyrosequencing.
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AQP5 expression and promoter methylation were monitored in both, dividing and nondividing EpH4 cells treated with Dex.
All methylation assays monitored incorporation of tritiated methyl groups in to DNA by using a modified ion-exchange filter binding assay [23].
Furthermore, noninvasive bioluminescence imaging of histone methylation sensors confirmed the potential of these sensors in monitoring histone methylation status in response to histone methyltransferase inhibitors in living animals.
An alternative strategy could be to monitor methylation in a noninvasively accessible biological fluid such as sputum or serum.
In this study, alterations in DNA methylation levels were monitored during benign, premalignant and malignant stages of ductal breast cancer development.
In addition, methylation was also monitored by site-specific RNase H cleavage (Yu et al, 1997) directed by a chimeric 2′- O-methyl RNA/DNA oligonucleotides to sites L2197 (5′-mAmCmUGGGCmAmGmAmAmAmUmCmAmCmAmUmU-3′) and L2288 (5′-mUmGmACGAGmGmCmAmUmUmUmGmGmCmUmAmC-3′).
Long-term growth potential and p16 promoter methylation in subsequent passages were also monitored.
The precise measurement of the LINE-1 methylation level may be a promising marker in monitoring hydatidiform moles before progression to GTN.
Then, we monitored GAD1 mCpG methylation in subclones of PCR products amplified from the immunoprecipitated DNA after bisulfite conversion.
The utilization of [H]-SAM in the assay allows monitoring methylation of each protein in the reaction mixture using SDS-PAGE followed by autoradiography.
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