Sentence examples for methylation in differentiated from inspiring English sources
Although they resemble imprinted genes in these respects, they fail to maintain differential methylation in differentiated somatic tissues due to de novo methylation of paternal alleles after implantation.
Taken together, our data suggest that G9a is not required for preferential targeting of DNMT3A/3B to silent methylated domains and faithful maintenance of DNA methylation in differentiated somatic cells.
These data indicate that DNA methylation in pluripotent stem cells is much more dynamic and error-prone than is maintenance methylation in differentiated cells.
Our data also show that while G9a is not required for maintenance of DNA methylation in differentiated cells, simultaneous targeting of G9a and DNMTs results in increased inhibition of cell proliferation and greater DNA hypomethylation.
Different HMTs including G9a, SUV39H1, EZH2 can direct DNA methylation via direct or indirect recruitment of DNMTs [68 70], which might be a mechanism for de novo methylation of DNA in ESC but appears to be nonessential for maintenance of methylation in differentiated cells [71].
We also tested whether the CpG island mapping PTTG1 proximal promoter evidenced a differential methylation pattern in differentiated thyroid cancer biopsies concordant to their PTTG1 immunohistochemistry status.
Embryonic stem cells (ESCs) generally rely on repressive histone modifications to silence retrotransposons, rather than DNA methylation as in differentiated cells.
The global DNA methylation patterns in differentiated ES cells with or without genistein treatment were analyzed with the MFMT assay, which involves using either HpaII or McrBC to dissect the methylation status of the end or interior CpGs for every MspI fragment, respectively (Figure S3).
Thus, it is essential to faithfully maintain DNA methylation patterns in differentiated cells through somatic divisions.
It is reasonable that developmental genes are shut off by DNA methylation in terminally differentiated cells.
We also indicated that group of genes categorized as developmental and transcription factor genes are shut off by DNA methylation in terminally differentiated astrocytes.
