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This work has produced important basic information on differences in methylation in different white blood cell types that will be critical for determining which biospecimens should be used for analysis in future studies.
Previous studies have shown global and gene specific differences in DNA methylation in different tissues in children prenatally exposed to tobacco smoking [ 11– 13].
In contrast to the constant levels of methylation in different stages of development, a large intertissue difference in global 5mC content was observed in some adult samples.
In this analysis, we divided all DMRs associated with genes into three groups (non-CpGi promoter, CpGi promoter, and intragenic CpGi), since as noted by others [ 37, 38], methylation in different gene locations may impact the gene expression regulation differently.
We showed that such cell class-specific scaling exponents are caused by different patchiness of DNA methylation in different cells.
The periphyton associated with freshwater macrophyte roots is the main site of Hg methylation in different wetland environments in the world.
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DNA methylation differences in different seed developmental stages and after exogenous application of ABA is suggestive of epigenetic regulation of gene expression.
Although the molecular mechanisms responsible for the differing methylation profiles in different tumour types remains unclear, gene methylation may have relevance in the clinical situation.
One limitation of DNA methylation analysis techniques is inability to differentiate heterogeneous methylation patterns in different cells present within samples [ 12].
However, the difference in the methylation levels in different tissues was not significant (p = 0.153).
We therefore examined male and female methylation levels in different human tissues to determine if tissue-specific methylation changes were frequent.
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