Sentence examples for methylation errors in from inspiring English sources
BPA also increased methylation errors in differentially methylated regions of maternally imprinted genes of oocytes in cultured preantral follicles of C57/BL6xCBA/Ca mice (Trapphoff et al. 2013).
Moreover, LD bisulfite sequencing also showed a significantly increased number of CpG methylation errors in GTL2, compared to LIT1 and PEG3.
We identified methylation errors in both the ICSI/IVF (n=39) and non-ICSI/IVF (n=39) groups indicating that ICSI was not responsible for these changes.
Thus, some genes may be more prone than others to stochastic methylation errors in sperm, e.g., by less stringent maintenance of the methylation imprints established in the male germline.
The most frequent methylation error in HOC was seen in the PEG1 DMR.
Indications for a link between ART and epigenetic regulation are that in the general population BWS is caused by a DMR CpG methylation error in 50-60% of the cases (Manipalviratn et al., 2009), while after ART almost all cases are related to hypomethylation of the maternal KCNQ1OT1 DMR (Table II).
To characterize a potential genetic cause for methylation errors described in oligozoospermia.
In another study testing possible transgenerational effects of tamoxifen in rats [ 171, 174], the offspring sired by tamoxifen-treated animals showed an increased incidence of embryonic resorptions, and resorbed embryos (but not normal ones) carried methylation errors similar to those detected in the sperm of exposed fathers.
We, and others, previously demonstrated that methylation errors are present at imprinted loci in the sperm of infertile men, particularly those with oligospermia.
Recently we developed a new high-throughput, high-resolution DNA methylation analysis method, bisulphite PCR-Luminex (BPL), using sperm DNA and demonstrated the effectiveness of this novel approach in rapidly identifying methylation errors.
Both sisters showed approximately 2% stochastic (single CpG) methylation errors: 10 of 531 CpG sites in the affected twin (excluding abnormal alleles) and 14 of 609 in the healthy twin were methylated.
