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Flash chromatographic separation gives 750 mg of 7-O galloyl catechin, 400 mg of catechin and 150 mg of methyl gallate from 4 g loaded fraction on ISCO.
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Methyl gallate isolated from TS was found to have a protective effect in hydrogen peroxide-induced oxidative stress and DNA damage in Madin-Darby canine kidney (MDCK) cells [ 9].
Methyl gallate is identified from EFNTE (70.44%) and BFNTE (12.77%) which is an antibacterial agent [ 43].
However, Jovanovic determined the two pKa values of free radical derived from methyl gallate to be 4.4 and 9.2 [12]; these pKa's for the free radical derived from gallic acid are 5.0 and 10.
This study is also reporting the existence of methyl gallate and pyrogallol for the first time from this species.
A number of compounds including retinoids, vitamins B and C, o-coumaric acid, kaempferol, methyl gallate, quercetin, afzelin, quercitrin, isoquercitrin, and rutin have been isolated from the leaves of TS [ 5].
Meanwhile lupane triterpenes; lupeol (7) and lupenone (8) together with methyl gallate (1) and a flavonol glycoside; quercitrin (12) have been isolated from the leaves.
Methyl gallate (C3) showed bioactivity slightly enhanced than Gallic acid.
The structure (C3) revealed to be methyl 3, 4, 5-trihydroxybenzoate or methyl gallate.
7-O-galloyl catechin (C1), catechin (C2), and methyl gallate (C3).
Although catechin and methyl gallate were evaluated previously for antioxidant potential by various methods [37].
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CEO of Professional Science Editing for Scientists @ prosciediting.com