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Triglyceride reacts with methanol to form methyl esters and glycerol.
The methoxides formed then reacted with the triglyceride to yield methyl esters and glycerols.
Therefore, the final product will be a mixture of epoxidized methyl esters and fatty acids [1].
The whole cell fatty acids are converted to methyl esters and analyzed by gas chromatography.
Identified peaks of the fatty acid methyl esters and their relative percentages are summarized in Table 3.
These two peaks confirmed the presence of methyl esters and its integration areas confirms the conversion on each sample.
Bio-diesel is a mixture of fatty acid methyl esters, and is produced by trans-esterification of renewable feed-stock.
The predicted response value for these conditions was 93.6% methyl esters and it was in agreement with the experimental value.
On the other hand, the D77I mutant had no detectable activity toward phenolic acid methyl esters and feruloylated arabinoxylan.
For constituents of fatty acid, methyl esters and fatty alcohol acetates were carried out by GC-MS after the saponification and derivatization of wax esters.
The FTIR spectra of the methyl esters and hydroxylated methyl esters were recorded using a Perkin Elmer FTIR system spectrum BX LR64912C.
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