Sentence examples for methods with subsequent from inspiring English sources
For the latter the wild-type genes (CelA2, BSLA and subtilisin Carlsberg) were cloned into the mutated backbone as described under Materials and Methods with subsequent rescreening in three 96-well microtiter plate measurements in which each construct was expressed 8 times per plate.
SDS-PAGE, blotting and the detection methods with subsequent densitometric quantification were performed as described earlier.
This consisted of Edward's background correction [ 40], global loess normalization [ 41], and statistical analysis using linear models and empirical Bayes methods with subsequent multiple testing correction using Benjamini-Hochberg's method [ 20] (adjusted p < 0.05 was considered significant).
Recombinant human dynein complexes were purified in a two‐step procedure (see Materials and Methods), with subsequent SDS PAGE analysis revealing bands for all six dynein subunits (Fig 1B).
Real-time PCR methods with subsequent melting curve analysis (MCA) using universal primers and fluorescent dyes have been successfully applied in malignant lymphoproliferative neoplasias in humans such as acute and chronic lymphocytic leukaemia, non-Hodgkin's lymphoma and cutaneous T cell lymphoma [ 13, 14].
Herein, hierarchical porous ZnO microspheres were fabricated by a facile hydrothermal method with subsequent calcination.
3D foam-like CoNiO2 on Cu foil was synthesized by a low-temperature hydrothermal method with subsequent annealing treatment.
Automata Networks (AN) were applied as a pre-processing method with subsequent ANN model development for Demirdöven Dam Reservoir.
A series of V2O5 TiO2 aerogel catalysts were prepared by sol gel method with subsequent supercritical drying with CO2.
The V2O5 self-assembled nanosheets (SANs V2O5) were synthesized by an additive-free ultrasonic method with subsequent thermal decomposition.
We prepare conformal-carbon-coated Zn2GeO4 nanowires (NWs) using a microwave-induced hydrothermal method with subsequent thermal decomposition.
