Lysates were spun at 14,000 x g at 4°C for 10 minutes and then analyzed for protein using the Bradford method with reagents from Bio-Rad Laboratories (Hercules, CA).
Immunohistochemistry was performed using the avidin biotin method with reagents from Biocare Medical (Walnut Creek, CA, USA).
We measured total protein using the Bradford method, with reagents from Bio-Rad Laboratories (HerCAles, CA).
Both laboratories measured BuChE activity using the Ellman method with reagents from Roche Diagnostics (Ellman et al. 1961).
Lysates were cleared by centrifugation, and protein concentrations were determined using the Bradford method with reagents from Bio-Rad (Hercules, CA).
Free T4 and TSH were measured in 100 μL plasma by an immunometric method with reagents from Roche Diagnostics (Mannheim, Germany), performed as a routine analysis at the Department of Clinical Chemistry, Lund University.
Analyses of U-Alb concentrations were performed at the Department of Clinical Chemistry, Sahlgrenska University Hospital (Gothenburg, Sweden) using an automated nephelometric immunochemical method with reagents and a calibrator from Beckman Coulter (Fullerton, CA, USA).
The reason may be multifold, including the Si component in the hybrid nanostructure, the growing method with especial reagents, and the existence of oxygen vacancy in ZnO nanowires [32] and carbon species as established in EDS spectra.
Fibrinogen concentrations were measured by using the Clauss method with commercial reagents (Mahsa-yaran, Tehran, Iran).
The DNA was isolated via an alkaline lysis method with Qiagen reagents (Qiagen, Inc., Valencia, CA) in a 96-well format using an Apricot pipettor (PerkinElmer Life and Analytical Sciences, Wellesley, MA).
