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This was in contrast to other scaled methods, which comprised at least two lysis steps for cellular extraction and subsequent lysis [ 26, 33] thereby increasing sample loss.
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It also points out that the digital integrated map system including urban local climates, energy consumption, and reference recommendations can be founded by blending and utilizing research methods, which comprises theoretical analysis, investigation, experiments, simulation, and GIS technologies.
In the section 2 of this article discretization of continuous distributions are discussed method wise including composite methods, which comprise two stages using two different methods in separate subsections.
To simplify the application of bioclimatic models, Chuine (2000) combined numerous major mechanistic models and developed a set of unified bioclimatic model calculation methods, which comprises two formulas to calculate the forcing and chilling requirements of plants.
We analyzed SOC using the modified Walkley Black method, which comprised boiling a soil-dichromate-sulfuric acid mixture for 5 min at 175 °C56.
It is generally agreed that the most outstanding achievement of linguistic scholarship in the 19th century was the development of the comparative method, which comprised a set of principles whereby languages could be systematically compared with respect to their sound systems, grammatical structure, and vocabulary and shown to be "genealogically" related.
To avoid using grid impedance estimation, [75] used a hybrid method which comprised passive damping and notch filter AD.
The following day, sections were incubated with secondary biotinylated donkey anti-rabbit or donkey anti-mouse antibodies (Dianova; 1 1,000) for 60 min at room temperature followed by the ABC method which comprised incubation with complexed streptavidin-biotinylated horseradish peroxidase.
The following day sections were incubated with secondary biotinylated donkey anti-rabbit, donkey anti-mouse or donkey anti-goat antibodies (Dianova 1 1,000) for 60 min at room temperature followed by the ABC method, which comprised incubation with complexed streptavidin biotinylated horseradish peroxidase.
The following day, sections were incubated with secondary biotinylated goat anti-rabbit antibodies (Dianova; 1 400) in TBS-2 % bovine serum albumin (BSA) for 60 min at room temperature followed by the ABC method which comprised incubation with complexed streptavidin-biotinylated horseradish peroxidase (Invitrogen).
In this study, we propose a novel approach to segmentation, the Real-time Unsupervised Segmentation (RUS) method, which comprises three phases.
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