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fitting point kernel methods were done for DLEBF computation.
Confirmation runs and comparisons among several optimization methods were done and indicated that the results fell within the respective confidence intervals for predictions, which corroborates the good adequacy of the proposal.
Further hybrid approaches combining quantitative and qualitative evaluation methods were done by [32, 79].
The methods were done as previously reported.
For subcellular fractionation, the methods were done as previously described.
These WB preparation methods were done for all microscopy techniques (described in the following paragraphs).
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The validation of the two methods was done according to the Directive 2002/657/EC.
Usually, classification of segmentation methods is done based on the approaches adopted and the domain of application.
The evaluation of the methods was done by means of objective function values, first feasible iteration and time consumption.
Comparison with other methods is done for both 90 and 100% of the frames.
An alternative way of calibration of the surface mapping methods was done with the reference sample (see "Methods" section).
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