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Quantities of the physical mixtures (25 mg) produced using the kneading and solvent evaporation methods were dissolved in 25 mL of phosphate buffer solution of pH 6.8.
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For the DR and gel-LC methods, EMVs were dissolved in 50 mM Tris HCl (pH 8.5), 0.2% SDS by boiling for 10 min. The samples were reduced (4 mM DTT for 10 min at 95°C) and alkylated (10 mM iodoacetamide, 30 min at room temperature in dark) and divided for DR and gel-LC analysis.
For dipping solutions of the LBL-SA method,PIA polymers were dissolved in DI water to the concentration of 0.01 M.
After estimating the total sugar contents of the cell wall extracts using the phenol-sulfuric acid method, the extracts were dissolved in DI water to a concentration of 0.2 mg mL-1.
In this method surfactant and cholesterol were dissolved in organic solvents and evaporated under reduced pressure by a rotary evaporator to produce a thin lipid film that was deposited on the wall of rotary flask.
The difference before and after enzymatic cleavage was then monitored by electrochemical method after the quantum dots were dissolved from the hybrids.
MALDI-TOF-MS measurements were performed on a Bruker REFLEX III-S (preparation method of sample: the samples were dissolved in dry THF, then mixed 1 1 (0.8 μl each) with a methanol solution of α-cyano-4-hydroxycinnamic acid as matrix); Elemental Analyses: Carlo-Erba Elemental Analyser 1104, 1106.
Crude mitochondria were isolated by differential centrifugation method and the P2 pellets were dissolved in 2.4 ml MirO5 (http://www.oroboros.at/index.php?id=524 857) buffer and 0.6 ml of this was used for measuring the respiration in Oxygraph.
In the citrate precursor method, Co(NO3 2·6H2O and Fe(NO3 3·9H2O were dissolved in deionised water (Milli-Q grade, Millipore Corp., Billerica, USA) to produce a solution with Co/Fe mole ratio of 1 2.
Parliaments were dissolved.
were dissolved in acetone.
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