Sentence examples for methods we sequenced from inspiring English sources
Here, using next generation sequencing methods, we sequenced the leaf transcriptome of O. officinalis, a CC genomic wild rice, and de novo assembled 68,132 unigenes based on approximately 23 million transcriptome reads.
Using Next Generation Illumina sequencing methods, we sequenced hepatic small RNA libraries from baboons differing in their LDL-C response to a high-cholesterol, high-fat (HCHF) challenge diet (low LDL-C, n = 3; high LDL-C, n = 3), resulting in 517 baboon miRNAs: 490 were identical to human miRNAs and 27 were novel.
As an alternative subtyping method, we sequenced two regions of SCCmec type III.
Initially, using the Sanger method, we sequenced a random selection of approximately 4000 recombinant plasmid clones from each of the two plasmid libraries.
By using the DGETP method, we sequenced 29.4 millions 20-mer tags across 7 distinct cDNA libraries obtained from 4th-stage larvae.
Using the library construction-based barcoding method, we sequenced exons 2 and 3 of HLA-A, HLA-B and HLA-C for each of 270 HapMap individuals (http://hapmap.ncbi.nlm.nih.gov/) (Table 1).
Depth of coverage plots per individual and per locus for the library construction method are shown in Additional Files 3, 4 and 5. Using the PCR-Based Barcoding method, we sequenced class I loci for 95 HapMap samples and produced a total of 45,398 reads.
Unlike previous methods, we sequence successive multiple libraries prepared with standard protocols, take two reads in a pair of large-insert PE reads as a tag, cluster other PE reads that have one end overlapped with it as local reads group, and locally assemble them to fill in the inner gap of the large-insert PE reads.
To assess the feasibility of using those conventional methods, we have sequenced the ITS gene of all isolates.
In order to evaluate these methods, we prepared sequencing libraries from three HapMap samples using both methods for the same target region and sequenced the libraries using a Genome Analyzer IIx instrument (Illumina).
After employing stringent success criteria (see 'Materials and methods') we obtained sequence-specificity data (8-mer scores and motifs) for 129 DBDs.
