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At the end of the process, after a number of data filtering steps (see Methods), we retained 5892 alignments for further analysis.
Among the autosomal SNPs that passed Quality Control analyses (see Methods), we retained the 36,320 SNPs polymorphic (Minor Allele Frequency or MAF above 0.01) in at least one West-African taurine and one West African zebu populations.
After the use of these methods we retained a total of four ecoregion clusters to which A. marginale sequences were unambiguously associated, and the rest were discarded because A. marginale strains were not isolated there.
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By this method, we retained 44 (22 hypertrophic and 22 non-hypertrophic) out of 55 tissue sites for further analysis.
After applying the filters described in the Materials and Methods section, we retained 731 protein hits.
For non-normally distributed variables we employed a simulation method, whereby we retained the phenotypic data (cognitive variables and affection status) and permuted the observed genotype among individuals using the R statistical package (http://cran.r-project.org).org
Using the selection methods discussed above, we retained in all of the final models SLCO1B1 rs11045819 and ABCC2 rs8187710.
After trimming, we performed additional quality score based filtering (see methods) and finally we retained 16.6 million reads (47% of the raw data) of 62 bp length corresponding to a total of 1.03 billion bp of sequence information (Table 1).
As described in Material and Methods, for each animal, we retained systematically the most parsimonious haplotype reconstruction (the reconstruction that requires the minimum number of recombination of ancestral haplotypes).
21 Using the method described previously 22 we retained four codes to define iatrogenic Cushing's syndrome: iatrogenic Cushing's syndrome, drug induced Cushing's syndrome, steroid facies, and cushingoid facies.
Figure 2 shows the grand-average ERD data with no cleaning and the same data cleaned by removing all non-neural sources for each method, except SSD for which we retained the five components with highest SNR.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com