Exact(2)
METHODS: We engineered Lewis lung cancer cells (LLCs) and bone marrow derived DCs to express tumor-associated antigen (TAA) ovalbumin (OVA) via lentiviral vector plasmid encoding OVA gene.
Methods: We engineered the food grade bacterium L lactis to secrete ovalbumin (OVA) and evaluated its ability to induce OVA-specific tolerance in OVA T-cell receptor (TCR) transgenic mice (DO11.10).
Similar(58)
Using the numerical plane wave expansion method, we engineer the absolute photonic band gap in modified lattices, achieved by addition of circular, elliptical, rectangular, square and hexagonal air hole or ring into the center of each lattice unit cell.
Methods: We have engineered the food-grade bacterium Lactococcus lactis to secrete bioactive murine TFF.
Methods: We genetically engineered the food-grade bacterium Lactococcus lactis to secrete the LcrV protein from the enteropathogenic species Yersinia pseudotuberculosis.
"We go down to, not just even the network layer, but even to the assembly level so that we can run some of the execution instructions very efficiently and some of the image processing on the GPU for phones," says Hu. "So on a high level it is a SLAM system, but the exact method and how we engineered it is novel for efficient mobile devices".
In conclusion, through a simple low-cost and high-output method-depositing Au film, we engineer the ordered array of nanopillars structure on the wing to form large-area high-performance SERS substrate.
We suggest methods to improve engineered PKS design by learning from laboratory-based selection; adoption of DNA design software and automation to build constructs and libraries more easily; tools for the expression of engineered proteins in a variety of heterologous hosts; and mass spectrometry-based high-throughput screening methods.
Furthermore, we described fabrication of myobundles in more detail, as follows, in the Materials and methods section: "Myobundles were formed by modifying our previously published methods for engineered rodent muscle tissues (Hinds et al., 2011; Juhas et al., 2014).
Myobundles were formed by modifying our previously published methods for engineered rodent muscle tissues (Hinds et al., 2011; Juhas et al., 2014).
We have recently developed methods to generate engineered biofilms, utilising centrifugation of recombinant E. coli onto poly-l-lysine coated glass supports instead of waiting for natural attachment to occur (Tsoligkas et al., 2011; 2012).
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