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Template DNA from SF EB and SF C. trachomatis PBMO as prepared by all previously described DNA extraction methods was subjected to PCR using four independently developed PCR primer sets and the commercially available Abbott LCX® (Abbott, Abbott Park, IL, USA).
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Quantitative and categorical data derived from the multiple sorting methods were subjected to multidimensional scaling analysis.
The extracts with higher yield from both methods were subjected to transesterification and GC MS analysis.
However, these methods are subjected to significant delays when implemented in real-time gait monitoring devices, orthoses, and FES systems.
The sequences not amplifiable by the above PCR methods were subjected to PCR with primers deduced from adjacent 5′ and 3′ sequences.
Hence, clusters of DNRs detected using spatiotemporal methods were subjected to a further epidemiological investigation in order to determine if any clusters represented a real outbreak.
The raw data obtained from 454 sequencing of the target enriched DNA (see Methods) were subjected to an analysis and filter process depicted in Figure 1.
All populations of both selection methods are subjected to 5000 generations of selective pressure withdrawal to simulate the disuse of specific antibiotics after prevalence of resistance.
Four probe sets, representing genes putatively involved in the fatty acid metabolism (listed in Methods) were subjected to real-time qRT-PCR analysis to validate the microarray data.
Both methods are subjected to permutation tests in order to establish per protein the significance of its distribution of CTL epitopes.
The sample of T. vorax, a species which preys on other tetrahymenas, is biased upward because all isolates that ate the T. thermophila mating type testers (see Methods) were subjected to barcode analysis and all were T. vorax.
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