The methods were optimised using a training set and their performance evaluated using a test set: this was repeated 100 times for different test and training set splits.
RNA isolation methods were optimised for high sensitivity, using only 3 μg of total RNA that were reverse transcribed using random hexamers, with the resulting cDNA hybridised after labelling to an oligonucleotide array.
Whole bones were collected at different sites of the skeleton; fixation and decalcification methods were optimised.
18 Until such methods are optimised and further research is performed in the area of maturation of iPSC-CMs, modelling of the more complex cardiovascular diseases including the cardiomyopathies will remain a major challenge.
The time is ripe to start on the iterative journey of producing guidance on quality and reporting standards as well as developing quality-assured learning resources to ensure that funding decisions, execution, reporting and use of these review methods is optimised.
The method was optimised with respect to three responses: resolution between peaks, theoretical plate count and the migration time of the EB peak.
A high pH LC/MS/MS method was optimised for rapid and sensitive analysis of both analytes in rat, dog and human plasma.
The fabrication method was optimised using the suspension feed rates which took into account the different deposition efficiencies of bioactive glasses and of HA.
A microwave-assisted extraction (MAE) method was optimised for extracting 2,4,6-trichloroanisole (TCA), 2,3,4,6-tetrachloroanisole (TeCA), pentachloroanisole (PCA), 2,4,6-trichlorophenol (TCP), 2,3,4,6-tetrachlorophenol (TeCP) and pentachlorophenol (PCP) from oak barrels.
In this work, the CVT method was optimised with respect to the parameter values of acquisition, reconstruction and analysis.
