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Methods: Utilizing restriction enzyme digestion, polymerase chain reaction amplification, and gel electrophoresis, the prkDTL5F11 plasmid was created by the fusion of distinct coding sequences for a single-chain GD2 targeting antibody (sc5F11) and truncated diphtheria toxin (DTA).
Genome reduction methods utilizing restriction digests (e.g., RAD-Seq, genotyping-by-sequencing; Davey et al., 2011) also produce thousands of loci, and have been effective in resolving phylogenetic relationships and patterns of introgression (e.g., Eaton and Ree, 2013).
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To further improve the contiguity and biological utility of the genome sequence, additional scaffolding methods utilizing independent genome and transcriptome assemblies were implemented.
In recent years several new screening methods utilizing bioluminescent cell-based biosensors have been designed demonstrating their utility towards dynamic monitoring of a variety of cellular functions.
Abacha and Khelifi investigated several query reformulation methods utilizing Mesh and DBpedia.
Besides, imaging inside turbid media has been enabled by other computational methods utilizing compressive sampling.
Mesoporous SnO2 has been previously prepared through various methods including sol-gel and sonochemical methods utilizing supramolecular templates.
Improving sedation methods utilizing precise control of preferred sedatives may increase patient satisfaction and practice efficiency.
Next, clinical and experimental injury and repair methods utilizing animal models are discussed.
An alternative to existing methods utilizing Newtonian balance equations or Hamilton's principle is thus provided.
In this review article, the focus is on active methods utilizing physical forces for cell sorting and manipulation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com