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Label-free methods utilize flow parameters of channels of various shapes and sizes to manipulate streamlines carrying cells and particles.
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We first report multiple skipping of dystrophin exons 6 and 8 in the DMD patient's cells and translation of the unified antisense PMO design from a DMD dog model to a human based on the MyoD-transduction method utilizing flow cytometry.
Results were considered positive if at least two out of three methods utilized (RT-PCR, flow cytometry, or immunofluorescence microscopy) were conclusive.
Here we describe a new method utilizing multicolor flow cytometry to isolate pure populations of EC from the skeletal muscle based on the antigens Sca-1, CD34, CD31 and CD45.
We then used these markers to develop a method utilizing multicolor flow cytometry to exclusively sort muscle derived venular, arterial and capillary EC which are all positive for Sca-1, CD31 and CD34 while excluding hematopoietic cells which express the common leukocyte antigen CD45.
Recognition methods utilize children's handwriting styles.
Different sciences use different terms, apply different methods, utilize different computer systems and technology.
The main methods utilized for calculating the flow field characteristics of aerostatic bearings include the finite element method (FEM) [25], the finite difference method (FDM) and CFD method [26].
The method utilizes open-flow respirometry, a technique that allows for the measurement of cellular respiration at steady state (e.g. O2 supply = O2 demand).
Due to AS's susceptibility to biodegradation and propensity to form calcium salts especially at the longer chainlengths, greatest confidence is given to the results from toxicity tests that utilize flow-through or static/renewal exposure methods with measured concentrations.
Figure 2 Process flow diagram describing the application of data, models, and methods utilized in this study.
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