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The predictive value of the selected biomarkers was validated by qPCR-based methods using tissue samples extracted from the 58 prostates and, separately, using 227 prostate core biopsies.
Next, the predictive value of the selected biomarkers was validated by qPCR-based methods using tissue samples extracted from resected prostates and, separately, in one out of 13-core biopsies from prostates that were negative and positive for PCa.
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Further work will evaluate in detail the maximum depth achievable with the proposed method using tissue samples or phantoms that mimic the acoustic attenuation of tissue.
Some studies evaluating the performance of microbial screening methods use tissues originating from animal medication experiments [ 47, 49, 56], but the production of incurred materials for each antibiotic at the appropriate concentration is a difficult and expensive task.
A common method used, tissue surface tensiometry (TST), applies an external force to an aggregate cultured under physiological conditions, and measures the resistance by the aggregate to the compressive force and changes in aggregate geometry.
In contrast, the whole mount method uses tissue fragments, making it possible to view large segments (and hundreds of endothelial cells) of essentially intact vessels.
Two immunohistochemical studies examined the prognostic significance of ALDH1 in pancreatic cancer, but their results conflict, perhaps because the evaluation methods differed (using tissue microarrays [ 15] vs. whole-mount tissue slides [ 16]).
Although experimental validation methods using soft tissue MR imaging phantoms can be developed, the data derived from these often suffers uncertainties similar to those present in the target soft tissue.
The ER stained slides were segmented by region classification methods using Definiens Tissue Studio (Munich, Germany).
Several recent basic research studies have described surgical methods for cardiac repair using tissue cardiomyoplasty.
Currently, models using tissue culture methods for measuring cell death in clonal cell lines, and in skin-equivalent models, have been accepted by regulators.
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