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We determined the level of A1M by automated nephelometric immunochemical methods using reagents and calibrator from Beckman Coulter.
Plasma total cholesterol and triglycerides were measured by enzymatic methods using reagents from Boehringer Ingelheim (Mannheim, Germany).
Apolipoprotein AI (apoAI) and apolipoprotein B (apoB) were assayed using immunoturbidimetric methods, using reagents supplied by ABX Diagnostics.
Serum concentrations of triglycerides and cholesterol were determined by enzymatic methods using reagents from Roche Diagnostics (Mannheim, Germany).
Plasma concentrations of triglycerides, cholesterol and phospholipids were determined by enzymatic methods using reagents from Boehringer-Mannheim (triglycerides and cholesterol; Mannheim, Germany) and Waco Chemicals (phospholipids; Neuss, Germany).
RNA isolated by benchtop and on-chip methods, using reagents from the High Pure Viral RNA Isolation Kit, were both analyzed by benchtop RT-PCR.
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Albumin was determined by an automated nephelometric immunochemical method using reagents and calibrator from Beckman Coulter (Fullerton, CA, USA).
Fibrinogen was determined with a modified Clauss method using reagents and an instrument (STA-R) from Diagnostica Stago according to the instructions of the manufacturer.
Urinary albumin concentrations were determined on fresh samples by an automated nephelometric immunochemical method using reagents and calibrator from Beckman Coulter (Fullerton, CA, USA).
In 1989, total cholesterol (TC) was measured enzymatically (CHOD-PAP method) using reagents from Boehringer (Manheim, Germany) and high density lipoprotein cholesterol (HDL-C) wasimilarlyly measured in the supernatant after precipitation of non HDL lipoproteins with phosphotungstate and MgCl.
Labeled cDNAs from cell samples for hybridization to the long-oligonucleotide and cDNA arrays were synthesized and labeled by the indirect amino-allyl method, using reagents and protocols supplied by the manufacturer.
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