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This is inherent to methods using chaotropic lysis buffers.
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Hexahistidine-tagged proteins can be purified under denaturing conditions using chaotropic concentrations of urea or guanidinium hydrochloride [ 14].
To address the unexpected assembly of scFv-A192 into nanoparticles, they were disrupted using chaotropic salt (6 M Guanidine) and renatured under dialysis.
An alternate way to bypass the membrane barrier is to use chaotropic agents or detergents [6].
The method uses chemical lysis by undisclosed lysis buffers containing chaotropic salt, enzymatic digestion by treatment with proteinase K and thermal lysis.
Another method uses mats.
Briefly, bacterial DNA was isolated using the chaotropic method (6).
One-step purification of soluble, functional recombinant FurA from the cyanobacterium Anabaena sp. PCC 7120 has been achieved using mild chaotropic conditions.
Samples were then solubilised using a chaotropic guanidinium isothiocyanate solution.
What methods were used?
The former uses a chaotropic buffer containing guanidine hydrochloride to selectively lyze human cells.
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