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The methods used, however, are not the ones described in Dr. Billingham's 1953 article.
Regardless of the samples or methods used, however, extraction of microbial DNA from soil was problematic, as a deep brown substance co-purified with the DNA.
The metabolically healthy overweight individuals were not at increased risk of mortality regardless of the methods used; however, this was not the case for the metabolically abnormal overweight individuals.
For long-term storage of individual microorganisms, cryopreservation and lyophilization are two major methods used; however, cryopreservation is generally preferred over lyophilization due to potential cell damage during the drying process [ 19].
Unfortunately, they did not provide full details of the statistical methods used; however, given the relative lack of within-study comparisons, it seems unlikely their methods can provide a valid analysis of treatment comparisons for such heterogeneous study data.
Differences in prevalence rates between countries can (partly) be attributed to differences in study population and research methods used; however, national differences such as climate or health care may also play a role.
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Our analysis confirmed several well known interactions, like the ones of NOD1/NOD2 and RIPK2, providing a good proof of principle for the method used, however, we observed surprisingly few interactions with adaptor molecules, especially ASC.
In a study by Rossi et al., following a single oral dose of 50 mg/kg ECH in male CD1 Swiss albino mice, levels of ECH were below the limit of quantitation (50 ng/mL) of the analytical method used; however, ECH was detected following an oral dose of 200 mg/kg.
Other methods are used, however, especially in the longer tours, in which some stages may be run as a time trial, with riders sent off at intervals, racing against the clock.
When the Klimisch method is used, however, GLP studies may be assigned to a higher reliability category by default [16].
The simulation method we used, however, does not take into account problems in gene tree reconstruction (the simulated gene trees are assumed to be accurate).
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