In this study, we investigated a method to reconstitute allograft with osteoregenerative factors.
We applied this method to reconstitute an endothelial cell monolayer to visualize the morphology of adherens junctions (AJs) which regulate permeability and integrity of the vascular barrier.
The key idea is to evaluate the Eshelby stress from discrete or atomistic simulations for a structured body, conceived as a numerical homogenization method to reconstitute the macroscopic continuum behavior in multiscale modelling approaches.
35 However, our method appears to reconstitute the function of tumor-reactive CD4+ Th1-lymphocytes and provide a large number of functioning tumor-reactive T-lymphocytes, resulting in large antitumoral responses in patients receiving large numbers of tumor-reactive T-cells.
A combination of freeze-thaw and dialysis methods were used to reconstitute the detergent-solubilized Light Harvesting 2 complex (LH2) of the purple bacterium Rhodopseudomonas acidophila strain 10050 into preformed egg phosphatidylcholine liposomes, without the need for extra chemical agents.
Using these methods, we were able to reconstitute complex actin network organizations, closely related to cellular architecture, to precisely direct and control their 3D connections.
Thus, several methods have been used to reconstitute interaction networks from multiple published datasets.
Overall, these methods require considerable time and labour to reconstitute the original native gene pool, and suitable nearby extant populations may not be available to carry them out.
The iTunes store does not warn about the permanence of its method of compression; once freeze-dried, there is no way to reconstitute the music into CD quality for playing through a good stereo.
However, it is difficult to reconstitute functional hetero-oligomeric complexes by this method.
The filtrate obtained by this method will have all the constituents except the enzyme and can thereafter be used to reconstitute the ligand solution.
