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For example, while Cas9 can be used to "knock out" genes through error-prone sequence disruption, methods to precisely insert or replace sequences are still in their infancy.
Engineering of cell fate through synthetic gene circuits requires methods to precisely implement control around native decision-making pathways and offers the potential to direct cell processes.
Fabricating a model of neural network in vitro is one of the promising methods to precisely assay the signal transmission and processing within the circuit as well as to examine behaviors of individual cells.
The development of synthetic polymer systems provides a second route to new materials: the concept of using a library of monomers and having the methods to precisely order them to design and produce a new polymer is a long-sought objective of polymer scientists.
These applications rely on methods to precisely control gene expression.
However, the contribution from veins can be relatively far downstream from active neurons, thereby limiting the ability of BOLD imaging methods to precisely pinpoint neural generators.
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Participants will be tested periodically for up to three years with angiograms and intravascular ultrasound, a three-dimensional imaging method to precisely define how much scar forms.
We then developed an adapted ribosome profiling method to precisely quantify and characterize the nature of mRNAs translated by distinct heterogenous ribosomes genome-wide.
We present graphite furnace atomic absorption spectrophotometric analysis (AAS) as a suitable method to precisely quantify hematoma volumes in rodent models of ICH.
At this scale, electrokinetic (EK) pumping offers a method to precisely meter liquid propellants under purely electrical control at pressures and flow rates well-suited for microthruster applications.
We present a method to precisely determine the exact copy number of CNP147 and examine in more detail the association with AMD.
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