Sentence examples for methods to normalise from inspiring English sources

In contrast, microarray analysis assesses the transcript abundance of tens of thousands of genes, thus it has required the application of statistical methods to normalise the distribution of signals and also requires correction for large samples sets, so called false discovery rate correction [ 3, 4].

138 There has also been a lack of uniformity of the comparator group, with controls consisting of healthy individuals in some studies and 'symptomatic control' patients in others and furthermore many of these studies used different methods to normalise miRNA data.

One of the main questions to address is effectively the relevance of the use of HKGs as an appropriate method to normalise RT QPCR results.

We are unable to recommend the use of Upright Standing in single leg stance as a SubMVC method to normalise EMG to if the muscles adductor longus and/or semitendinosus are the intended muscles of investigation.

Initially, a set of calibration STM strains with VNTR loci of different sizes were initially sequenced to verify the method and to normalise the raw data to be obtained from prospective MLVA typing.

Delta Ct methods were used to normalise the expression of the chosen genes of interest against the average of the endogenous control genes.

These issues are not new – the discussion of the optimal methods by which to normalise for body-mass effects began at least a century ago (Rubner, 1883; Kleiber, 1932; Kleiber, 1961).

The standard curve method was used to normalise the gene expression data.

Default parameters were applied, global scaling method was used to normalise data (TGT value set at 100).

The median-of-ratios method was used to normalise the data to correct for unequal sequencing depth; this procedure was implemented as a default in DESeq2.

The ΔΔCp method was applied to normalise to the expression of TERT mRNA, using both the expression of β actin and a non-tumour brain tissue sample.