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Also the development of the methods to genotype all these SNPs using genotyping arrays was an important prerequisite.
We used the VNTR-7 and VNTR-16 methods to genotype the 189 clinical isolates of M. tuberculosis, following the protocol described by Zhang [15].
We used PCR methods to genotype transgenic mice using a pair of primers (see Supplementary file 1).
To estimate the false discovery and false negative rates (FDR and FNR) of our SNP calls, we analyzed the same samples using Affymetrix 6.0 SNP arrays (Methods) to genotype for common SNPs.
The recent development of methods to genotype directly from sequence data, such as genotyping-by-sequencing (GBS) [ 19], can decrease the cost of marker-based selection for production traits compared to SNP array platforms.
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We have developed a method to genotype variable number of tandem repeats (VNTRs) and insertion/deletion polymorphisms using an integrated microfluidic chip-based system.
Real-time PCR and fluorogenic probes were combined in a simple, rapid and sensitive method to genotype murine breeding stocks and their progeny for a point mutation.
Sequence-based typing (SBT) is a discriminatory method to genotype Legionella pneumophila serogroup strains.
Another genome-scale method to genotype common retrotransposon insertion polymorphisms (RIPs) to identify genotype-phenotype associations uses array-based technology.
In these cases, discovery and genotyping methods based on SNPs may be confounded and molecular cytogenetics remains the only method to genotype these inversions.
The method to genotype DArT markers was said to be technologically challenging [ 25], but in our case, it gave reasonable results with high reproducibility.
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