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To enhance the potential of NV− for temperature sensing [6 11], we have developed methods to conjugate FNDs with other nanoparticles such as gold nanorods (GNRs) to form multi-functional nanohybrids [12].
This discussion will include preparation of unique prosthetic groups and methods to conjugate these prosthetic groups to the biomolecules.
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Meanwhile, Lai et al. [ 35] utilized this dopamine treatment method to conjugate BMP on titanium for the first time; the covalent conjugation was performed under alkaline conditions as suggested by Lee et al. [ 28].
CDAP activation of bacterial nanocellulose is shown to be a convenient method to conjugate extracellular proteins to the scaffold surfaces.
We present herein a simple and robust method to conjugate human Annexin V (ANX), which avidly binds to cells in the earliest, reversible stages of apoptosis, to superparamagnetic iron oxide (SPIO) nanoparticles, which serve as an MRI-detectable contrast agent.
We developed a method to conjugate biotin to the surface of magnetic nanoparticles by using a simple and easy to apply chemical approach.
Zimmerman et al. used this method to conjugate monomethyl auristatin F (MMAF) with the trastuzumab, which afforded a potent ADC (Zimmerman et al., 2014).
To test this, we developed a method to conjugate PlnD1 onto electrospun PCL fibers and tested the resulting modified PCL scaffold for increased binding and delivery of BMP-2 as well as bioactivity in inducing in vitro osteogenesis.
Here, we describe a simple and efficient method to conjugate free reducing glycans with proteins using a commercially available chemical linker.
To exclude the possibility that the AVE-1642 chemicalinkagege with QDs was unstable in serum and resulted in the dissociation of the antibody with QDs, we developed an alternative linkage method to conjugate AVE-1642 with QDs.
This is because PEGs of different lengths can characteristically block the pore current; this soluble and flexible polymer is unlikely to form a folded structure with DNAs or affect DNA structures, and copper(I -catalyzed clI -catalyzedy provides a simple and rapid method to clickgate PEG with DNA in high yield.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com