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We compared the efficacy of these methods to bacterial culture by quantitatively measuring levels of P aeruginosa in serial dilutions, in reconstituted skin samples and 21 burn wound biopsy samples.
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They are useful in illustrating both the difficulty of using standard culture methods to assess bacterial communities and in revealing that dragon mouths harbor many bacterial species that could potentially spread as an oral epidemic under the right life history conditions.
Using these proteomic methods to analyze bacterial pathogens has substantial promise, but no example yet exists of global protein expression analysis of a bacterial pathogen growing inside its natural host or in an animal model.
There is an increasing interest in developing new methods to reduce bacterial adhesion onto polymeric materials used in biomedical implants.
This study showed the benefits of employing several different complementary methods to investigate bacterial enzyme activity.
This technology provides a framework for methods to manipulate bacterial genomes using CRISPR-nickase systems.
Part of the debrided tissue was also analyzed using culture methods to evaluate bacterial diversity of the biofilms.
We have previously developed combinatorial mutagenesis and selection methods to improve bacterial promoter systems for diverse purposes.
PCR-based methods to detect bacterial and fungal DNA have yet to be validated in larger cohorts [ 8, 93, 105, 120, 164].
Also new dressing materials based on silk fibers and enhanced methods to reduce bacterial burden, for example, cold argon plasma, might help to fasten wound healing.
We assessed the risk for hand contamination and compared the efficacy of five hand hygiene methods to remove bacterial contamination in a tertiary Vietnamese hospital.
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