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After removing contamination from TruSeq adapters and the low quality portion of the reads using the methods mentioned above, we applied three methods to assemble the transcriptomes: (1) de novo assembly by Trinity [ 37], (2) reference-based assembly by TopHat [ 38] (v2.0.10) and Cufflinks [ 39] (v2.2.1), and (3) reference-guided assembly by Trinity.
The fourth additional de novo assembly used the Extender program as above on 1,000 new random reads, and we only used this assembly to identify SVMPs, which were difficult for other methods to assemble.
Specifically, chromophore units with protected-amino groups and one carboxylic group are described, together with methods to assemble these units using peptide chemistry.
He has also pioneered methods to assemble these "building blocks" into unique structures that have impacted and created new opportunities in areas ranging from electronics and computing to biology and medicine.
Another recent challenge, issued by the Defense Advanced Research Projects Agency (Darpa), was aimed at identifying new methods to assemble shredded documents — or potential vulnerabilities in the military's current document shredding practices.
To overcome these difficulties, we have developed an efficient internal pore architecture design system based on triply periodic minimal surface (TPMS) unit cell libraries and associated computational methods to assemble TPMS unit cells into an entire scaffold model.
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This paper describes ultrasound enhanced electrostatic batch assembly (U2EBA) as a low-cost, batch microassembly method to assemble 3D microsystems.
This paper presents a reasonable method to assemble the ITER VV.
The results not only provide a method to assemble high performance PECD, but also disclose its potential for commercialization.
In this work, we develop a facile electro-deposition method to assemble Co ultrathin shell on the Co3O4 nanowires forming Co3O4@Co core/branch arrays (CBAs).
We present an effective method to assemble the PSs in specified regions by hydrophiling the substrate in designed regions for the nanophotonics with nanostructures in well-definite regions.
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