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A final round of PCR is then employed in both methods to amplify the target product using outside primers.
Another approach to improving the sensitivity of cell detection is to use polymerase chain reaction (PCR) methods to amplify DNA specific for cancer cells.
Methods to amplify complete mitochondrial genomes rapidly and efficiently from microgram quantities of tissue of single individuals are, however, not always available.
We used standard PCR methods to amplify 93.2% of the genome excluding the single-stranded ends of each segment [ 7], and purified PCR reactions for sequencing using ExoSAP-It (US Biological, Swampscott, MA).
Many genotyping technologies use PCR-based methods to amplify a region surrounding the variant locus, and differ by the marker type and the technology platform used to assay for it.
The development of PCR methods to amplify cassette arrays using primers in the 5′-CS and 3′-CS of Class 1 integrons and the equivalent regions of other MRI and the increase in complete sequencing of resistance plasmids have led to an explosion in the number of cassette array sequences in databases such as GenBank.
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This model illustrates the residual QSAR method to amplify asymptotically the computed toxicity towards the observed carcinogenicity (Figure 1).
A method to amplify long genomic regions (up to ∼12.3 kb) from pestiviruses in one RT-PCR is described.
We applied a whole genome amplification method to amplify DNA from buccal cell samples stabilized using FTA® technology.
In PCR-based WGA methods, degenerate oligonucleotide-primed PCR (DOP-PCR) is the most widely used method to amplify the entire genome [37, 38].
This design guarantees uniform amplification across the whole genome and also endows TTAS a versatile method to amplify different types of DNA material.
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