The enormous putative off-target sites are labor-intensive to be validated experimentally, thus motivating bioinformatics methods for rational design of sgRNA and prediction of its potential off-target effects.
However, previous studies reported substantial off-target activities of CRISPR system in human cells, and the enormous putative off-target sites are labor-intensive to be validated experimentally, thus motivating bioinformatics methods for rational design of CRISPR system and prediction of its potential off-target effects.
The results presented show that Kernel Regression is an efficient solution for such scenarios, thus motivating further usage of Kernel methods in wireless modeling.
However, for research focused on studying differential expression of known genes, sequence information at single-base level may not be required, thus motivating the development of alternative methods for high-throughput, yet highly accurate quantification of gene expression.
It has often been assumed, however, that most compensatory mutations occur at positions that are close in space, thus motivating the development of computational methods for identifying co-evolving positions as distance constraints in protein structure prediction (Göbel et al., 1994).
However, this single image method yielded inaccurate 3D reconstructions as depth estimations were about 10 mm, thus motivating the need of using multiview geometry for accurate 3D reconstruction.
We are thus motivated to solve a dual problem[28].
We are thus motivated to make a direct comparison of the results produced by these theories by use of the same numerical method to solve physically the same wave diffraction problems.
These resources can become revenue for authorities and thus motivate profit-seeking punishment.
Platforms introduce teams to create a sense of competition and thus motivate members to contribute.
