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As explained in the Methods, the sequence numbers of the L and M opsin gene groups could be underrepresented and the nucleotide diversity and divergence values for the gene groups could therefore be overestimated.
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In principle, there are two kinds of computational methods: the sequence-based methods and network-based methods.
Compared to RFLP/SSR and array-based SFP genotyping methods, the sequence-based method produces a map of the highest density.
Compared with another sensitivity analysis method, the Morris method, the sequence of the importance of parameters is consistent.
In order to increase the specificity of this method, the sequence length of the stem-loop RT primer to individual miRNAs was extended to 8 nucleotides complementary to the 3' end of the miRNA.
By the threading method, the sequence with an unknown structure is fitted into a variety of existing structures.
The main technical difference between the GMO methods is the sequence of the primers and probes.
Sequence-based methods explore the sequence conservation in proteins under the assumption that ligand-binding site sequences are conversed in the evolution process.
Using the BSAS method, the sequencing run produced 8.22 million reads, and 6.54 million reads were mapped after read merging, quality trimming, and elimination of PhiX control reads.
Upon alignment (see Methods), the VH sequence matched VH1-69*01 (100% identity, Additional file 4: Figure S2).
The first method divides the sequence into blocks whose length is selected graphically by means of the "statistical inefficiency".
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