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Using previously described methods, the expression of two stage-specific genes, Odam (most highly expressed during maturation stage) and Enam (most highly expressed during secretory stage), were checked by real-time PCR to ensure the accuracy of dissections and quality of total RNA collected from each individual sample [ 13, 16].
According to different cooperative forwarding methods, the expression of differs for AF cooperation by the relay and is given as follows [12]: (6).
By utilizing the Fourier expansion and the Laplace transform methods, the expression for the dynamic response of displacement is obtained in a general form which is applicable to general dynamic pressures.
In most of the existing eigenvalue-based methods, the expression for the decision threshold and the probabilities of detection and false-alarm are calculated based on the asymptotical distributions of eigenvalues.
To confirm the specificity of this increase, we examined, in addition to GAPDH (our loading control, see Western Blot section in MATERIALS AND METHODS), the expression of another unrelated protein, histone H2b (His H2b) (Figure 1C).
However, due to the fact that several of the fsr-genes had been excluded from the data analysis as a result of the number of functional spots in the latter strain (see Materials and methods), the expression of fsrB was verified by real time quantitative PCR (QPCR; Figure S3).
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With the k-means method, the expression levels for each condition (time and infection status) of the SLA/PrV differentially expressed probes set were clustered in three groups.
With this method the expression levels of Kir4.1 and aquaporin-4 were quantified in glutamine synthetase positive Müller cells.
In the MICs method, the expression products of cecropin and moricin were diluted to final concentrations of 20, 10, 5, 2.5, 1.25, 0.625, 0.313 or 0.157 µmoL/L, and added to bacteria at logarithmic phase.
By this method, the expression measure is given in log2 base.
Depending on the method, the expression may be estimated using probe level expression or some computed probe set level expression index.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com