Uniaxial extension is the simplest and most widely utilized method among the ex vivo testing methods.
In this view, quick, easy and quantitative methods for the ex vivo determination of pERK in PBMC have been developed on the basis of cytofluorimetric techniques.
We then correlated the resulting expression values to multiple adipose-relevant measures (Supplementary file 1H [ Gubelmann et al., 2014], 'Materials and methods'), including the ex vivo differentiation potential of human SVF from subcutaneous adipose tissue biopsies, as previously described (Meissburger et al., 2011).
To validate our method, the ex-vivo irradiated samples were analyzed to monitor the changes due to irradiation.
Here, we report a novel and highly potent two-step culture method for the ex vivo generation of functional NK cells for clinical application in the treatment of patients with AML and other malignancies.
Two other organotypic methods, the Ex-Vivo Eye IrriTeston TEVEITEVEIT) based on isolated rabbit corneas (Spöler et al. 2007; Frentz et al. 2008) and the Porcine Corneal Ocular Reversibility Assay (PorCORA) based on isolated porcine corneas (Piehl et al. 2010, 2011), have been proposed to directly address reversibility/persistence of effects, but neither has yet undergone a formal validation study.
There is no denying that Ferguson's methods work – the ex-players lined up to praise his man-management skills and 30 years of trophies speak for themselves – but he has made mistakes beyond letting Jaap Staam go, the only error he admitted to.
It was confirmed that the bottom water/gas injection system was an effective corium cooling method in the ex-vessel core catcher to suppress the quick release of steam.
The application of this method for imaging the ex situ water intrusion into the porous network of GDLs is explored using absorption and phase contrast methods.
These initial data were used to establish a method to proceed with the ex vivo component of the clinical study.
Instead he is seeking to prosecute opposition MPs on charges of bribery, which smacks of the methods preferred by the ex-president he ousted, Maumoon Abdul Gayoom.
